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GenScript corporation pcmv-dr8.91 g193486
Pcmv Dr8.91 G193486, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv-dr8%2E91+g193486/pcmv+dr8+91+g193486/pmc11874419__pnas__2403891122__sapp-149-12-16
Average 90 stars, based on 1 article reviews
pcmv-dr8.91 g193486 - by Bioz Stars, 2026-09
90/100 stars

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Transfection:

Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption
Article Snippet: For lentiviral generation, HEK293FT were transfected with shRNA in pLKO.1 backbone and viral packaging vectors, pCMV-dR8.91 (GenScript G193486) and envelope plasmid pVSV-G (GenScript G178153), as well as the corresponding mission shRNA or non-targeting shRNA at a 10:9:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific e3411668019) per manufacturer’s guidelines.

shRNA:

Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption
Article Snippet: For lentiviral generation, HEK293FT were transfected with shRNA in pLKO.1 backbone and viral packaging vectors, pCMV-dR8.91 (GenScript G193486) and envelope plasmid pVSV-G (GenScript G178153), as well as the corresponding mission shRNA or non-targeting shRNA at a 10:9:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific e3411668019) per manufacturer’s guidelines.

Plasmid Preparation:

Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption
Article Snippet: For lentiviral generation, HEK293FT were transfected with shRNA in pLKO.1 backbone and viral packaging vectors, pCMV-dR8.91 (GenScript G193486) and envelope plasmid pVSV-G (GenScript G178153), as well as the corresponding mission shRNA or non-targeting shRNA at a 10:9:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific e3411668019) per manufacturer’s guidelines.



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GenScript corporation pcmv-dr8.91 g193486
Pcmv Dr8.91 G193486, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv-dr8%2E91+g193486/pcmv+dr8+91+g193486/pmc11874419__pnas__2403891122__sapp-149-12-16
Average 90 stars, based on 1 article reviews
pcmv-dr8.91 g193486 - by Bioz Stars, 2026-09
90/100 stars
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